In part one, perform a gram stain on bacteria cultures to determine which are gram-negative and which are gram-positive. Hypothesis: Based on your research, write a detailed hypothesis predicting the answer to the question.Įxperiment: An experiment to test your hypothesis will need two parts. Observe/Gather Data: Do some research to find information about antibiotics and gram staining, so that you can make an informed hypothesis. Question: Will four common antibiotics (Penicillin, Ampicillin, Neomycin, and Erythromycin) have the same effect on both gram-negative and gram-positive bacteria? Gram staining helps doctors make a diagnosis, but can it also help suggest a cure? What is the relationship between gram classification and antibiotic use? Do common antibiotics interact differently with gram-positive and gram-negative bacteria? Answer these questions through experimentation. For example, the bacteria that causes scarlet fever is gram-positive, while that which causes typhoid or cholera is gram-negative. Even the simple determination that a bacteria specimen is gram-positive or gram- negative can direct a doctor in diagnosis, as different bacteria cause different diseases. In the process, he discovered that bacteria could be divided into two different groups - one that retained a stain, called ‘gram-positive,’ and one that didn’t, called ‘gram-negative.’ His unique method for identifying these two groups became the first step in any bacterial identification process. In 1884 Hans Christian Gram, a Danish bacteriologist, attempted to find a universal stain that would work with all bacteria. Ever heard about gram staining and antibiotics? Here’s how it started.
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